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assay detected antibody
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Antibody
Practically, several immunodiagnostic methods based on detection of complex antigen-antibody are used to diagnose infectious diseases, for example ELISA, immunofluorescence, Western blot, immunodiffusion, immunoelectrophoresis, and Magnetic immunoassay. ... "Enzyme-linked immunosorbent assay (ELISA)". -
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ELISA
(1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form. ... Assay -
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Western blot
The confirmatory HIV test employs a Western blot to detect anti-HIV antibody in a human serum sample. ... Enzyme assay - Protein assay - Secretion assay -
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Assay
A wide range of cellular secretions (say, a specific antibody or cytokine) can be detected using the ELISA technique. ... Methods of assay of precious metals -
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Immunoprecipitation
By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates. ... Assay -
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HIV test
Antibody tests may give false negative (no antibodies were detected despite HIV being present) results during the window period, an interval of three weeks to six months between the time of HIV infection and the production of measurable antibodies to HIV seroconversion. ... The enzyme-linked immunosorbent assay (ELISA), or enzyme immunoassay (EIA), was the first screening test commonly employed for HIV. -
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Hepatitis C
Hepatitis C testing begins with serological blood tests used to detect antibodies to HCV. ... "An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.". -
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Enzyme assay
These types of assay can be extremely sensitive, since the light produced can be captured by photographic film over days or weeks, but can be hard to quantify, because not all the light released by a reaction will be detected. The detection of horseradish peroxidase by enzymatic chemiluminescence (ECL) is a common method of detecting antibodies in western blotting. -
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Secretion assay
The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). ... Assay -
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JN-QC-SPOT TB
In one of the Epidemiology study the LISA assay has a specificity to detect antibodies to TB is 97%, the sensitivity is 70-80%. ... 4. Thomas M. Daniel 1988, Antibody and Antigen detection for the immunodiagnosis of Tuberculosis; Why not?
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assay detected antibody