Finding:

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   Enzyme assay

   These types of assay can be extremely sensitive, since the light produced can be captured by photographic film over days or weeks, but can be hard to quantify, because not all the light released by a reaction will be detected. ... Typical enzymes are active in salt concentrations of 1-500 mM.
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   Bradford protein assay

   An exception of note is elevated concentrations of detergent. ... The nonlinearity issue of the Bradford assay may be solved by measuring the ratio of OD at 595 to 450 nm.
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   Assay

   A wide range of cellular secretions (say, a specific antibody or cytokine) can be detected using the ELISA technique. ... Methods of assay of precious metals
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   Comet assay

   The damage usually detected are single strand breaks and double strand breaks. ... The comet assay is an extremely sensitive DNA damage assay.
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   ELISA

   These samples of known antigen concentrations will constitute a standard curve used to calculate antigen concentrations of unknown samples. ... Secretion assay
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   SNP genotyping

   This incorporated base is detected and determines the SNP allele (Goelet et al. 1999; Syvanen 2001). ... Genotyping by Oligonucleotide Ligation Assay (OLA)
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   Electrophoretic mobility shift assay

   If the starting concentrations of protein and probe are known, the affinity of the protein for the nucleic acid sequence may be determined. ... This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein-nucleic acid complex.
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   Norovirus

   There is an inherited predisposition to infection and people whose blood type can be detected in their saliva are more often infected. ... These assays are very sensitive and can detect concentrations as low as 10 virus particles.
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   Protein purification

   For example, imidazole (commonly used for purification of polyhistidine-tagged recombinant proteins) is an amino acid analogue and at low concentrations will interfere with the bicinchoninic acid (BCA) assay for total protein quantification. ... Each band can be detected using stains such as Coomassie blue dye or silver stain.
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    Inborn error of metabolism

    Guthrie bacterial inhibition assay (detected a few amino acids in excessive amounts in blood) The dried blood spot can be used for multianalyte testing using Tandem Mass Spectroscopy (MS/MS).

Explore the following pages on Powerset:

• Enzyme assay,
• Bradford protein assay,
• Assay,
• Comet assay,
• ELISA,
• SNP genotyping,
• Electrophoretic mobility shift assay,
• Norovirus,
• Protein purification,
• Inborn error of metabolism