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assay detected level

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Results for "assays detect levels"

Guthrie test The Guthrie test is a semiquantitative assay designed to detect elevated blood levels of the amino acid phenylalanine, using the ability of phenylalanine to facilitate bacterial growth in a culture medium with an inhibitor.

Guthrie test The Guthrie assay is sensitive enough to detect serum phenylalanine levels of 180-240 μmol/L (3-4 mg/dL).

Polyomavirus ELISA-based assays for large T-Antigen and small T-Antigen are also used to detect the level of expression and thus whether reactivation has occurred .

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    Comet assay

    The Single Cell Gel Electrophoresis assay (also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. ... The damage usually detected are single strand breaks and double strand breaks.
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    Enzyme assay

    These types of assay can be extremely sensitive, since the light produced can be captured by photographic film over days or weeks, but can be hard to quantify, because not all the light released by a reaction will be detected. ... Level of crowding, large amounts of macromolecules in a solution will alter the rates and equilibrium constants of enzyme reactions, through an effect called macromolecular crowding.
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    Luciferase

    Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. ... Assay Drug Dev Technol 5 (1): 127–36. doi:10.1089/adt.2006.053.
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    Guthrie test

    The Guthrie assay is sensitive enough to detect serum phenylalanine levels of 180-240 μmol/L (3-4 mg/dL). ... When an elevated level of phenylalanine is detected, the laboratory notifies the infant's physician, who explains the result to the family and arranges testing to determine the cause of the high phenylalanine levels.
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    Nerve guidance conduit

    Furthermore, nerve regeneration with these conduits has yet to reach the level of functional recovery seen with nerve autografts. ... PSA-expressing Schwann cells did obtain enhanced motility as demonstrated in a gap bridging assay and after grafting in postnatal forebrain slice cultures.
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    ELISPOT

    Following a wash to remove any unbound biotinylated antibody, the detected cytokine is then visualized using an avidin-HRP, and a precipitating substrate (e.g., AEC, BCIP/NBT). ... An animated depiction of the cytokine ELISPOT assay process
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    Republic of China

    Due to the relocation of many manufacturing and labor intensive industries to the PRC, unemployment also reached a level not seen since the 1970s oil crisis.
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    Clonogenic assay

    For example, at a dose of 0 or 1 gray of radiation, 500 cells might be plated, but at 4 or 5 grays 2500 might be plated, since very large numbers of cells are killed at this level of radiation and the effects of the specific treatment would be unobservable. ... "Clonogenic assay of cells in vitro".
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    Von Willebrand disease

    Type 2N can only be diagnosed by performing a "factor VIII binding" assay. ... Decreased levels of vWF are detected (10-45% of normal, i.e. 10-45 IU).
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    Protein purification

    Some proteins function as receptors and can be detected during purification steps by a ligand binding assay, often using a radioactive ligand.

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