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ELISA
Enzyme-Linked ImmunoSorbent Assay, also called ELISA, Enzyme ImmunoAssay or EIA, is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. ... The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody which is linked to an enzyme through bioconjugation. -
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Tetramer assay
A tetramer assay is used to detect the presence of antigen specific T-cells. ... The tetramer can be detected by flow cytometry via the fluorescent label. -
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Comet assay
The comet assay is a versatile technique for detecting damage and with adjustments to the protocol can be used to quantify the presence of a wide variety of DNA altering lesions (damage). The damage usually detected are single strand breaks and double strand breaks. -
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SNP genotyping
If only the first probe’s fluorophore wavelength is detected during the assay then the individual is homozygous to the wild type. ... Ligation does not generally occur in the presence of a mismatched 3' base. -
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Bradford protein assay
In SDS concentrations above CMC, the detergent associates strongly with the green form of coomassie dye, causing the equilibrium to shift, thereby producing more of the green form, which causes an increase in the absorbance at 595 nm independent of protein presence. ... Assay -
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AFP-L3
The L3 isoform is specific to malignant tumors and its detected presence can serve to identify patients whom need increased monitoring for the development of HCC in high risk populations (i.e. chronic hepatitis B & C and/or liver cirrhosis). ... AFP-L3% assay -
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DNase footprinting assay
A DNase footprinting assay is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. ... The cleavage pattern of the DNA in the absence of a DNA binding protein, typically referred to as free DNA, is compared to the cleavage pattern of DNA in the presence of a DNA binding protein. -
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Electrophoretic mobility shift assay
When these conditions are not met a second band might be seen in lane 3 reflecting the presence of free DNA or the dissociation of the DNA-protein complex. ... This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein-nucleic acid complex. -
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Lowry protein assay
The product becomes reduced to molybdenum/tungsten blue and can be detected colorimetrically by absorbance at 650nm. ... Presence of strong acids or ammonium sulfate can interfere with the assay. -
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History of radar
The system detected presence of ships up to 3 km, and he planned to extend its capability to 10 km. ... Eventually the Germans gave up trying to bomb them.
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assay detected presence