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assay measured antibody
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Antibody
In clinical immunology, levels of individual classes of immunoglobulins are measured by nephelometry (or turbidimetry) to characterize the antibody profile of patient. ... "Enzyme-linked immunosorbent assay (ELISA)". -
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ELISA
This antibody is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal. ... Secretion assay -
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Assay
An assay is a procedure where a property or concentration of an analyte is measured. ... A wide range of cellular secretions (say, a specific antibody or cytokine) can be detected using the ELISA technique. -
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Immunoprecipitation
By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates. ... Assay -
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Enzyme assay
Amounts of enzymes can either be expressed as molar amounts, as with any other chemical, or measured in terms of activity, in enzyme units. ... MTT assay -
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Western blot
Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. ... Enzyme assay - Protein assay - Secretion assay -
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Survivin
In a luciferase reporter assay, if the promoter is active, the luciferase gene is transcribed and translated into a product that gives off light that can measured quantitatively and thus represents the activity of the promoter. ... B. Humoral Antibody Response -
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Thymidine kinase
The monoclonal antibody has been bound (conjugated) to alkaline phosphatase (an enzyme). ... This assay gives a considerably more sensitive determination. -
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Immunohistochemistry
Alternatively, the antibody can also be tagged to a fluorophore, such as FITC, rhodamine, Texas Red or Alexa Fluor (see immunofluorescence). ... Assay -
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Electrophoretic mobility shift assay
An antibody that recognizes the protein can be added to this mixture to create an even larger complex with a greater shift. This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein-nucleic acid complex.
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assay measured antibody