Finding:

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   Enzyme assay

   A large number of different methods of measuring the concentrations of substrates and products exist and many enzymes can be assayed in several different ways. ... Assay
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   Protein

   Complementary to the field of structural genomics, protein structure prediction seeks to develop efficient ways to provide plausible models for proteins whose structures have not yet been determined experimentally. ... Assay
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   Bradford protein assay

   It is therefore necessary to use a standard (e.g. BSA-- Bovine Serum Albumin) whose protein closely matches the measured protein in composition, but systematic error(different amino acid composition for all proteins) should be taken into account when performing the assay.
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   Assay

   An assay is a procedure where a property or concentration of an analyte is measured. ... Assays for studying interactions of proteins with DNA include:
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   Protein purification

   If the protein has a distinguishing spectroscopic feature or an enzymatic activity, this property can be used to detect and quantify the specific protein, and thus to select the fractions of the separation, that contains the protein. ... Some proteins function as receptors and can be detected during purification steps by a ligand binding assay, often using a radioactive ligand.
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   Comet assay

   The concept underlying the SCGE assay is that undamaged DNA retains a highly organized association with matrix proteins in the nucleus. ... Olive, P.L.; Wlodek, D. & Banath, J.P. (1991): DNA double-strand breaks measured in individual cells subjected to gel electrophoresis.
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   Immunoprecipitation

   RIP is also an in vivo method in that live cells are exposed to formaldehyde in order to create cross-links between RNA and RNA-binding proteins. ... Assay
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   Magnesium transporters

   The transport of other cations by the Alr1p protein was assayed by the inhibition of yeast growth. ... The TM topologies of the Mrs2p and Lpe10p proteins have been assessed using a protease protection assay and are shown in the figure.
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   ATP-binding cassette transporter

   Transported radioactive substrates or labeled fluorescent dyes can be directly measured, or in an indirect set up, the modulation of the accumulation of a probe substrate (e.g. fluorescent dye, like Rho123, or calcein) can be determined in the presence of a test drug. ... The calcein assay is the proprietary assay of SOLVO Biotechnology.
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    Two-hybrid screening

    The most common screening approach is the yeast two-hybrid assay. ... If the two proteins do not interact, there is no transcription of the reporter gene.

Explore the following pages on Powerset:

• Enzyme assay,
• Protein,
• Bradford protein assay,
• Assay,
• Protein purification,
• Comet assay,
• Immunoprecipitation,
• Magnesium transporters,
• ATP-binding cassette transporter,
• Two-hybrid screening