Finding:

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   Polymerase chain reaction

   PCR is used to amplify specific regions of a DNA strand (the DNA target). ... A 1971 paper in the Journal of Molecular Biology by Kleppe and co-workers first described a method using an enzymatic assay to replicate a short DNA template with primers in vitro.
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   Variants of PCR

   There are many choices for the all-important DNA polymerase used in PCR: ... "Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands".
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   SNP genotyping

   Several developments have extended the original Invader assay. ... The double-stranded PCR products are denatured using heat and formaldehyde to produce ssDNA.
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   History of polymerase chain reaction

   (This article assumes familiarity with the terms and components used in the PCR process.) ... At this same time the original OR assay technique is replaced with the more general ASO method.
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   Bisulfite sequencing

   Alternatively, nested PCR methods can be used to enhance the product for sequencing. ... ↑ Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
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   Mycobacterium ulcerans

   The immobilized DNA is washed to remove inhibitors and used directly as a template for IS2404 PCR. ... "Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection".
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   DNase footprinting assay

   A DNase footprinting assay is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. ... For example, the DNA fragment of interest may be PCR amplified using a 32P 5' labelled primer, with the end result being many DNA molecules with a radioactive label on one end of one strand of each double stranded molecule.
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   Immunoprecipitation

   The identity and quantity of the DNA fragments isolated can then be determined by PCR. ... Enzyme assay - Protein assay - Secretion assay
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   Allele specific oligonucleotide

   ASO analysis is only one of the methods used to detect genetic polymorphisms. ... The RFLP assay was briefly adapted to the use of oligonucleotide probes, but this technique was quickly supplanted by ASO analysis of polymerase chain reaction (PCR) amplified DNA.
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    Tuberculosis diagnosis

    The Mantoux test is now used. ... This is a heterogeneous group of tests that use the polymerase chain reaction (PCR) technique to detect mycobacterial nucleic acid.

Explore the following pages on Powerset:

• Polymerase chain reaction,
• Variants of PCR,
• SNP genotyping,
• History of polymerase chain reaction,
• Bisulfite sequencing,
• Mycobacterium ulcerans,
• DNase footprinting assay,
• Immunoprecipitation,
• Allele specific oligonucleotide,
• Tuberculosis diagnosis