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assay used luciferase

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Tricine The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.

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    Luciferase

    Luciferase can be used in blood banks to determine if red blood cells are starting to break down. ... Assay Drug Dev Technol 5 (1): 127–36. doi:10.1089/adt.2006.053.
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    Enzyme assay

    These assays can be used to measure reactions that are impossible to assay in any other way. ... Another example is the enzyme luciferase, this is found in fireflies and naturally produces light from its substrate luciferin.
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    Survivin

    Different colour fluorescence is used to distinguish compartment from survivin. ... In a luciferase reporter assay, if the promoter is active, the luciferase gene is transcribed and translated into a product that gives off light that can measured quantitatively and thus represents the activity of the promoter.
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    Bioreporter

    Nonspecific lux bioreporters are typically used for the detection of chemical toxins. ... ↑ Legler, J. et al. (1999) Development of a stably transfected estrogen receptor-mediated luciferase reporter gene assay in the human T47D breast cancer cell line.
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    Tricine

    The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase. ... ↑ Webster, J. J., and Leach, F. R., "Optimization of the firefly luciferase assay for ATP."
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    Promega

    Such dual-luciferase reporter assays are used to monitor activity of G-protein coupled receptor pathways, investigate modulation of gene activity through antioxidant response elements, and identify molecules that enhance STAT-1-dependent gene expression in a high-throughput screen. ... ASSAY and Drug Development Technologies. 5, 127–36.
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    GUS reporter system

    Several kinds of GUS reporter gene assay are actually available, depending of the substrate used: the term GUS staining refers to the most common of these, an histochemical technique. ... Other competing systems are based on e.g. luciferase, GFP, beta-galactosidase, chloramphenicol acetyltransferase (CAT), alkaline phosphatase.
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    Cytotoxicity

    Cytotoxicity can also be monitored using the MTT or MTS assay. ... Such ATP-based assays include bioluminescent assays in which ATP is the limiting reagent for the luciferase reaction.
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    Reporter gene

    Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins; examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, and the enzyme luciferase, which catalyzes a reaction with a luciferin to produce light. ... Reporter genes can be used to assay for the activity of a particular promoter in a cell or organism.
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    Quorum sensing

    A variety of different molecules can be used as signals. ... For instance, the bioluminescent luciferase produced by V. fischeri would not be visible if it were produced by a single cell.
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