Finding:

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   Zinc finger chimera

   The specificity of ZFPs selected by phage display, is tested using a multitarget enzyme-linked immunsorbant assay (ELISA). ... These assays are repeated using different target oligonucleotides.
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   Electrophoretic mobility shift assay

   This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein-nucleic acid complex. ... The use of different oligonucleotides of defined sequence allows the identification of the precise binding site by competition (not shown in diagram).
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   Allele specific oligonucleotide

   It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. ... The use of synthetic oligonucleotides as specific probes for genetic sequence variations was pioneered by R. Bruce Wallace, working at the City of Hope National Medical Center in Duarte, California.
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   SNP genotyping

   The same oligonucleotides are used in the following step as immobilized single-base extension primers on a microarray (Krjutskov et al. 2008). ... Oligonucleotide ligase assay
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   Gene synthesis

   The mutation problem could be solved by shorter oligonucleotides used to assemble the gene.
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   HIV test

   The enzyme-linked immunosorbent assay (ELISA), or enzyme immunoassay (EIA), was the first screening test commonly employed for HIV. ... Special oligonucleotides are added which bind to viral RNA and to certain oligonucleotides bound to the wall of the vessel.
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   Branched DNA assay

   The assay entirely relies on hybridisation as principle. ... Several different short single-stranded DNA molecules (oligonucleotides) are used in a branched DNA-assay.
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   History of polymerase chain reaction

   To achieve this, he pioneers many of the techniques needed to make and use synthetic DNA oligonucleotides. ... At this same time the original OR assay technique is replaced with the more general ASO method.
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   Survivin

   Another experiment on 4003 involved assessing any biological or cytotoxic effect 4003 down-regulation of survivin mRNA has on A549 cells using the MTT assay. ... To further see whether survivin played a key role in sensitization of the cancer cells to cytotoxic drugs, survivin antisense oligonucleotides were used to knock down any survivin mRNA and thus its possibility to be translated is also eliminated. siRNAs for survivin are basically complements in sequence to the mRNA sequence encoding survivin.
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    Oligonucleotide synthesis

    They are most commonly used as primers for DNA sequencing and amplification, as probes for detecting complementary DNA or RNA via molecular hybridization, and for the targeted introduction of mutations and restriction sites, allowing for the synthesis of artificial genes. ... Oligonucleotides are chemically synthesized using phosphoramidites.

Explore the following pages on Powerset:

• Zinc finger chimera,
• Electrophoretic mobility shift assay,
• Allele specific oligonucleotide,
• SNP genotyping,
• Gene synthesis,
• HIV test,
• Branched DNA assay,
• History of polymerase chain reaction,
• Survivin,
• Oligonucleotide synthesis