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assay used probes

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Real-time polymerase chain reaction This potentially allows for multiplexing - assaying for several genes in the same reaction by using specific probes with different-coloured labels, provided that all genes are amplified with similar efficiency.

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    Allele specific oligonucleotide

    It acts as a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler Dot blot assay. ... The RFLP assay was briefly adapted to the use of oligonucleotide probes, but this technique was quickly supplanted by ASO analysis of polymerase chain reaction (PCR) amplified DNA.
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    SNP genotyping

    This is addressed somewhat by using several redundant probes to interrogate each SNP. ... The primer extension method is used in a number of assay formats.
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    Enzyme assay

    UV light is often used, since the common coenzymes NADH and NADPH absorb UV light in their reduced forms, but do not in their oxidised forms. ... MTT assay
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    DNA microarray

    It is used to assay the expression of alternative splice forms of a gene. ... These may be compared to other genes within a sample or to reference "normalizing" probes used to calibrate data across the entire array and across multiple arrays.
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    Test probe

    A test probe (test lead, test prod) is a physical device used to connect electronic test equipment to the device under test (DUT). ... Passive scope probes
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    Real-time polymerase chain reaction

    Using fluorescent reporter probes is the most accurate and most reliable of the methods, but also the most expensive. ... Polymerisation of a new DNA strand is initiated from the primers, and once the polymerase reaches the probe, its 5'-3-exonuclease degrades the probe, physically separating the fluorescent reporter from the quencher, resulting in an increase in fluorescence.
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    Oxygen radical absorbance capacity

    ORAC levels of various foods These are "old" data from the ORAC assay used before 2004 and should be viewed only as qualitative rankings.
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    Fluorescent in situ hybridization

    Differently-coloured probes can be used for the detection of translocations. ... Enzyme assay - Mass spectrometry - Chromatography - Flow cytometry - Blood bank - Microbiological culture - Serology
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    Comet assay

    For calibration, hydrogen peroxide is usually used to provide a standardized level of DNA damage. ... The comet assay is an extremely sensitive DNA damage assay.
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    Chemotaxis assay

    For detection of cells general staining techniques (e.g. trypan blue) or special probes (e.g. mt-dehydrogenase detection with MTT assay) are used.

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